IKB-β (Y188) polyclonal antibody

Product Specifications

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified. The regulation of IκBβ and IκBε is similar to that of IκBα. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics. IKK phosphorylation of IκBβ occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; NFKBIB; IKBB; TRIP9

Swiss Prot

Q15653

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human IKB-β.

Conjugation

Unconjugated

Related Pathways

Death Receptor Signaling, Inhibition of Apoptosis, NF-kB Signaling, T cell receptor signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:100~1:500

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 52, 58 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IKB-β (Y188) polyclonal antibody detects endogenous levels of IKB-β protein.

Applications Notes

Western blot (WB) analysis of IKB-β (Y188) polyclonal antibody at 1:500 dilution Lane1:EC9706 whole cell lysate (60ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (60ug) Lane4:HEK293T whole cell lysate (60ug) Lane5:CT-26 whole cell lysate (40ug) Lane6:U-87MG whole cell lysate (60ug)