PPARγ (S112) polyclonal antibody

Product Specifications

Background

Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Peroxisome proliferator-activated receptor gamma; Short name=PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3; PPAR γ; PPARγ

Swiss Prot

P37231

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human PPARγ.

Conjugation

Unconjugated

Related Pathways

MAPK Erk in Growth and Differentiation Pathway (, MTOR signaling

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:100~1:500

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 65 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PPARγ (S112) polyclonal antibody detects endogenous levels of PPARγ protein.

Applications Notes

Western blot (WB) analysis of PPARγ (S112) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (30ug) Lane2:CT-26 whole cell lysate (30ug) Lane3:The Stomach tissue lysate of Mouse (30ug) Lane4:The Heart tissue lysate of Rat (30ug)