ATG3 polyclonal antibody
Product Specifications
Background
This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
ATG3; APG3; APG3-LIKE; APG3L; PC3-96
Swiss Prot
Q9NT62
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human ATG3.
Conjugation
Unconjugated
Applications
WB IP
Dilution
WB,1:500 - 1:2000 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATG3 polyclonal antibody detects endogenous levels of ATG3 protein.
Applications Notes
Western blot (WB) analysis of ATG3 pAb at 1:2000 dilution Lane1:K562 whole cell lysate (30ug) Lane2:THP-1 whole cell lysate (30ug) Lane3:BV2 whole cell lysate (30ug) Lane4:PC12 whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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