ATG3 polyclonal antibody

Product Specifications

Background

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

ATG3; APG3; APG3-LIKE; APG3L; PC3-96

Swiss Prot

Q9NT62

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human ATG3.

Conjugation

Unconjugated

Applications

WB IP

Dilution

WB,1:500 - 1:2000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 40 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ATG3 polyclonal antibody detects endogenous levels of ATG3 protein.

Applications Notes

Western blot (WB) analysis of ATG3 pAb at 1:2000 dilution Lane1:K562 whole cell lysate (30ug) Lane2:THP-1 whole cell lysate (30ug) Lane3:BV2 whole cell lysate (30ug) Lane4:PC12 whole cell lysate (30ug)