Syk (P342) polyclonal antibody

Product Specifications

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells. Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors. It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas. Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl. Tyr352 of Syk is involved in the association of PLCγ1. Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK

Swiss Prot

P43405

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human Syk.

Conjugation

Unconjugated

Related Pathways

Immune Cell Signaling, B cell receptor signaling

Applications

WB IHC IF IP

Dilution

WB: 1:2000~1:5000 IHC: 1:50~1:200 IF: 1:100~1:500 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Syk (P342) polyclonal antibody detects endogenous levels of Syk protein.

Applications Notes

Western blot (WB) analysis of Syk (P342) polyclonal antibody at 1:2000 dilution Lane1:A431 whole cell lysate (30ug) Lane2:HEK293T whole cell lysate (30ug) Lane3:THP-1 whole cell lysate (30ug) Lane4:PMVEC whole cell lysate (30ug) Lane5:CT-26 whole cell lysate (30ug)