GEMIN2 mouse monoclonal antibody

Product Specifications

Background

This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

SIP1; SIP1-delta; gem nuclear organelle associated protein 2

Swiss Prot

O14893

Reactivity

Human, Mouse, Rat

Immunogen

Full length human recombinant protein of human GEMIN2 (NP_003607) produced in E.coli.

Conjugation

Unconjugated

Applications

IHC, WB

Dilution

WB 1:2000, IHC 1:150

Purity

The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG1

Applications Notes

Western blot (WB) analysis of GEMIN2 mouse monoclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (30ug) Lane2:HEK293T whole cell lysate (30ug)