Propidium (Iodide)

Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

Product Specifications

CAS Number

[25535-16-4]

UNSPSC

12171500

Hazard Statement

H341

Target

DNA/RNA Synthesis; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage; Others

Applications

COVID-19-immunoregulation

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Propidium_Iodide.html

Purity

99.69

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 3.57 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]

Molecular Formula

C27H34I2N4

Molecular Weight

668.39

Precautions

H341

References & Citations

[1]Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43 (10) :1031-8.|[2]A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.|[3]Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.