Anti-human IgE
Mouse monoclonal antibody to IgE
Product Specifications
Reactivity
Human
Immunogen
Myelomatous human serum
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography
Concentration
1ug/ul
Dilution
Detection of total IgE level and IgE antibodies in ELISA. Recommended pairs (coating - conjugate) : 5D4 – 4F4, 4G7– BE5. MAb E411 can be used for coating. MAb 4F4 can be used for IgE purification by affinity chromatography. MAb XTE4 can be used in Western blotting.
Purity
MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Detection of total IgE level and IgE antibodies in ELISA. Recommended pairs (coating - conjugate) : 5D4 – 4F4, 4G7– BE5. MAb E411 can be used for coating. MAb 4F4 can be used for IgE purification by affinity chromatography. MAb XTE4 can be used in Western blotting.
Tested Applications
ELISA, WB
Host or Source
Mouse
Preservative
PBS, pH 7.4 with 0.05% sodium azide.
Isotype
IgG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 IgG2a for MAbs 4F4, 5D4 and E411
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