Anti-Clathrin (619-638)

A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine.

This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy ie a dilution range of 1: 100 to 1: 400. For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended.

This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy ie a dilution range of 1: 100 to 1: 400. For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended.