Anti-glutathione peroxidase 1 (GPx-1, 175-194)

A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 (GPx-1) conjugated to diphtheria toxin has been used as the immunogen.

This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000 depending on tissue and detection method. For western blotting a dilution range of 1: 10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 is recommended. The optimal dilution should be determined by the end user. This antiserum has extremely high titre, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes.

This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000 depending on tissue and detection method. For western blotting a dilution range of 1: 10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 is recommended. The optimal dilution should be determined by the end user. This antiserum has extremely high titre, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes.