Anti-58K Golgi protein (Internal)

Recommended dilutions WB: 1/500~2000 Predicted molecular weight: 59 kDa. Observed band size : 120 kDa ï1⁄4ˆWhy is the actual band size different from the predicted? Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.ï1⁄4‰ Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Recommended dilutions WB: 1/500~2000 Predicted molecular weight: 59 kDa. Observed band size : 120 kDa ï1⁄4ˆWhy is the actual band size different from the predicted? Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.ï1⁄4‰ Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.