Anti-Interleukin-6 (IL-6) Mouse
Rabbit polyclonal antibody to Interleukin-6 (IL6)
Product Specifications
Reactivity
Mouse
Immunogen
Highly pure (>98%) recombinant mouse IL-6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Affinity purified
Dilution
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (0.1 ng/ml), a concentration of 0.012 - 0.014 μg/ml of this antibody is required. ELISA:To detect mIL-6 by direct ELISA (using 100Âμl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6. Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Purity
Affinity purified
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (0.1 ng/ml), a concentration of 0.012 - 0.014 μg/ml of this antibody is required. ELISA:To detect mIL-6 by direct ELISA (using 100Âμl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6. Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Tested Applications
ELISA, WB
Host or Source
Rabbit
Preservative
PBS buffer, pH 7.4. Reconstitute in sterile water at a concentration of >0.2 mg/ml.
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