BCECF AM - green fluorescent pH indicator
BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings. Excitation: 450, 500 nm | Emission: 530 nm | Purity: >95%
Product Specifications
Synonyms
2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein Acetoxymethyl Ester
Weight
0,5 lbs
Shipping Conditions
Overnight
Shipping Width
10 in
Shipping Height
1,5 in
Shipping Length
12 in
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