Anti-VaChT Transporter Antibody

Mouse Monoclonal Antibody specific to VAChT Transporter

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

VAChT, Solute carrier family 18 member 3

Gene Name

SLC18A3

NCBI Gene ID

UniProt

Q16572

Cellular Locus

Membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 521-532 (CEDDYNYYYTRS) of human VAChT (accession no. NP_003046)

Target Antigen

Vesicular acetylcholine transporter

Target

VaChT Transporter

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Involved in acetylcholine transport into synaptic vesicles. {PubMed:8071310}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of 55kDa is detected. The molecular weight of glycosylated VAChT is 70kDa. Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat VAChT.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.