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MCP-1, Mouse, mAb ECE.2 Antibody

Mouse monoclonal antibody to MCP-1

Product Specifications

Product Name Alternative

C-C motif chemokine 2, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2, Sigje Gene name: Ccl2

Reactivity

Mouse

Cross Reactivity

Human - No

Immunogen

Synthetic peptide corresponding to residues 102-130 of mouse MCP-1

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

F: Sections (6 um) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37°C or o/n 4 °C and then incubated with 2 μg/ml antibody for 2h at 37°C. W: Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 μg/ml) for 20 min at RT.

Tested Applications

IHC-Fr, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Isotype

Rat IgG1

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