Anti-CCR5 (NT) Antibody

Rabbit Polyclonal Antibody specific to CCR5 (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD antigen CD195

Gene Name

CCR5

Gene ID

1234

NCBI Gene ID

UniProt

P51681

Accession Number

NP_000570

Cellular Locus

Cell membrane, Multi-pass membrane protein

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 6-20 of human CCR5.

Target Antigen

C-C chemokine receptor type 5

Target

CCR5 (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Infectious Disease

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed:30713770). {PubMed:10383387, PubMed:11323418, PubMed:30713770, PubMed:8639485, PubMed:8663314, PubMed:8699119}.; (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1. {PubMed:10383387, PubMed:21763489, PubMed:8649511, PubMed:8649512, PubMed:9632396}.

Additionnal Information

Immunoblotting: use at 1:1,000-1:2,000 dilution. <br><br>Positive control: Whole cell lysate from THP-1 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human CCR5 (40 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Human immunodeficiency virus (HIV) and related viruses require coreceptors in addition to CD4 to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, Bonzo, GPR1, and V28, have been identified as HIV coreceptors. Among them, CCR5 is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains. CCR5 is required for infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-1alpha, and MIP-1beta are the ligands for CCR5 and prevent infection by macrophage-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV binding sites. Messenger RNA for CCR5 is expressed in lymphoid cells and tissues.