SIRP alpha Recombinant Rabbit Monoclonal Antibody [JE36-20]
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB1. Binds PTK2B.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Swiss Prot
P78324 Human, P97797 Mouse, P97710 Rat
Cellular Locus
Membrane
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within Human SIRP alpha aa 395-504 (Cytoplasmic).
Isotype
IgG
Clone
JE36-20
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, FC
Positive Control
THP-1 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, human lymph node tissue, human spleen tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000 ;IHC-P: 1:1,000 ;FC: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 55 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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