MBL, Human, mAb 3E7 Antibody

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

FC: cells were incubated with 1.0 μg/ml MBL in PBS containing 1%BSA (w/v), 0.01% NaN3 (w/v) and 10 mM CaCl2 for 30 min at 0°C. The cells were washed with the same buffer, and incubated with antibody clone 3E7, 6.5 μg/ml. After 30 min at 0°C, cells were washed and incubated with phycoerythrin (PE) -conjugated goat F (ab€) 2 anti-mouse Ig (Ref.3) . IHC-F: Tissue sections embedded in ornithine carbamoyltransferase compound were frozen in acetone dry ice and immersed in Tris-buffered saline (TBS) three times for 5 minutes. Treatment in 0.5% hydrogen peroxide in methanol solution has been done to quench endogenous peroxidase activity (Ref.2) . FS: Pretreatment of sheep erythrocytes with antibody 3E7 suppressed hemolysis in a dose dependent manner (Ref.1) .