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Galectin-3, Human, mAb B2C10 Antibody

Mouse monoclonal antibody to Galectin-3

Product Specifications

UniProt

P17931

Reactivity

Human

Cross Reactivity

Mouse - Yes

Immunogen

Recombinant human Galectin-3

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS containing 0.1% bovine serum albumin.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IHC: Endogenous peroxidase activity was blocked by incubation with 1.1% H2O2 for 10 minutes. Nonspecific binding of the second antibody was avoided by preincubation with normal rabbit serum for 15 minutes. FS: 10 mg/ml of B2C10, but not an isotype-matched control mAb, completely inhibited monocyte migration induced by galectin-3 at all concentrations examined (ref. 2) .

Tested Applications

FC, Functional Studies, IA, IHC-Fr, PS, WB

Preservative

Quantities >500 μg will be sent without preservatives and are carrier-free.

Isotype

Mouse IgG1

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