C5b-9, Rat, mAb 2A1 Antibody
Mouse monoclonal antibody to C5b-9
Product Specifications
Product Name Alternative
Membrane attack complex, MAC, C5b-9, TCC, Terminal Complement Complex
Reactivity
Rat
Immunogen
Rat C5b-9
Clonality
Monoclonal
Conjugation
Unconjugated
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IA: plates were coated with 30μg/ml in 50mM carbonatebuffer, pH10.6 for 16h at 4°C. (Ref.1) IHC-P: Tissue sections fixed in formalin were pretreated with protease type XXIV for 10 minutes at 37°C before incubation (Ref.6) . IHC-F: Tissue sections were fixed in acetone for 10 minutes at room temperature before incubation (Ref. 3) .
Tested Applications
FC, IA, IF, IHC-Fr, PS
Preservative
Quantities >500 μg will be sent without preservatives and are carrier-free.
Isotype
Mouse IgG1
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