Fibroblasts, Mouse, mAb ER-TR7 Antibody
Mouse monoclonal antibody to Fibroblasts
Product Specifications
Reactivity
Rat
Immunogen
Mouse thymic stromal cells
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Dilution
For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:20.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
F: Sections were stained using an indirect immunoperoxidase method (Ref.1) . FC: Splenocytes were incubated with ER-TR7 for 30' (Ref.4) . IF: Acetone or PFA fixed cells were quenched with 50mM NH4Cl for 30', blocked and permeabilized with 1.5% goat serum/0.1% saponin in PBS for 45' at RT. Incubation of ER-TR-7 in block and perm solution for 45' at RT. Specific staining was detected with a fluorescent conjugated oat anti-rat IgG (Ref.5) . P: Formalin fixed paraffin sections were deparafined, hydrated in ethanol and stained with ER-TR7 for 3'; at RT (Ref.3) .
Tested Applications
FC, IF, IHC-Fr, PS
Preservative
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rat IgG2a
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