Fmoc-L-Lys (Pentynoyl-DIM) -OH

Fmoc-L-Lys (Pentynoyl-DIM) -OH is a click chemistry reagent containing an azide. Fmoc-L-Lys (Pentynoyl-DIM) -OH can be used as a SPPS building block for the “helping hand” strategy for purification of highly insoluble peptides. Solubilizing residues are attached to the Lys side-chains using Click-chemistry. The solubilizing tag can be removed with 1M hydrazine or hydroxylamine solution[1]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.