Human CD39 (R138A, M139A, E142K) Protein, His Tag (active enzyme)

CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells (Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.2 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P49961-1 (R138A, M139A, E142K)

Expression Region

Thr 38 - Val 478

Host

HEK293

Target

CD39

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD39 / ENTPD1 (R138A, M139A, E142K), His Tag (Cat. No. CD9-H52H7) at 1 μg/mL (100 μL/well) can bind Anti-CD39 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).