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Nitrate Colorimetric Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Plant Physiology Series

Assay Principle

Nitric oxide (NO) is chemically active and is rapidly metabolized in the body to nitrite (NO2-) and nitrate (NO3-), and NO2- is further converted into NO3-. In this kit, NO3- was reduced to NO2- by reductant agent, and reacts with chromogenic agent to produce color substance. The nitrate content can be determined at 540 nm.

Assay Performance Time

1 h 30 min

Sample Type

Serum; Plasma; Urine; Tissue; Cell; Cell culture supernatant

Detection Range

0.68-100.00 μmol/L

Sensitivity

0.68 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Inter CV

7.8-9.3%

Intra CV

1.2-3.8%

Detection Instrument

Microplate reader

Frequently Asked Questions

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