Pseudouridine 5'-triphosphate

pseudouridine-5’-triphosphate (Pseudo-UTP) is one of the most commonly used modified nucleoside for the polymerase-mediated synthesis of RNA molecules. Compared with uridine-containing unmodified mRNAs, the application of pseudouridine-containing modified mRNAs exhibits better nuclease stability, immunogenicity, and translational properties[1]..

[1]Shanmugasundaram M, Senthilvelan A, Kore AR. Gram-Scale Chemical Synthesis of Base-Modified Ribonucleoside-5'-O-Triphosphates. Curr Protoc Nucleic Acid Chem. 2016;67:13.15.1-13.15.10. |[2]Borchardt EK, Martinez NM, Gilbert WV. Regulation and Function of RNA Pseudouridylation in Human Cells. Annu Rev Genet. 2020;54:309-336. |[3]Anderson BR, Muramatsu H, Nallagatla SR, et al. Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation. Nucleic Acids Res. 2010;38(17):5884-5892. |[4]Morais P, Adachi H, Yu YT. The Critical Contribution of Pseudouridine to mRNA COVID-19 Vaccines. Front Cell Dev Biol. 2021;9:789427. Pu|[5]Muthian Shanmugasundaram, et al. An Efficient Protection-Free One-Pot Chemical Synthesis of Modified Nucleoside-5'-Triphosphates. Nucleosides Nucleotides Nucleic Acids. 2016 Jul 2;35(7):356-62.