NHC-triphosphate

NHC-triphosphate is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form[1]. NHC-triphosphate is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA[1][2].

Product Specifications

CAS Number

[34973-27-8]

UNSPSC

12352005

Target

CHIKV; Endogenous Metabolite; Enterovirus; HCV; SARS-CoV; Topoisomerase

Type

Reference compound

Related Pathways

Anti-infection; Cell Cycle/DNA Damage; Metabolic Enzyme/Protease

Applications

COVID-19-anti-virus

Field of Research

Infection

Assay Protocol

https://www.medchemexpress.com/nhc-triphosphate.html

Purity

99.80

Solubility

H2O : 160 mg/mL (ultrasonic)

Smiles

O[C@H]1[C@H](N(C=C/C2=N/O)C(N2)=O)O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@H]1O

Molecular Formula

C9H16N3O15P3

Molecular Weight

499.16

References & Citations

[1]Stuyver LJ, et al. Ribonucleoside analogue that blocks replication of bovine viral diarrhea and hepatitis C viruses in culture.Antimicrob Agents Chemother. 2003 Jan;47 (1) :244-54.|[2]Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C (Powder, protect from light, stored under nitrogen)

Scientific Category

Reference compound1

Clinical Information

No Development Reported

Citation 01

ACS Bio Med Chem Au. 2022 Oct 24;2 (6) :565-585.|Commun Biol. 2022 Feb 22;5 (1) :154.|J Biol Chem. 2021 Jul;297 (1) :100770.|Nucleic Acids Res. 2021 Jan 8;49 (D1) :D1113-D1121.|ACS Sens. 2022 May 27;7 (5) :1564-1571.|bioRxiv. 2021 Jul 21:2021.07.21.453274. |J Control Release. 2025 Sep 18:387:114245.|Nat Struct Mol Biol. 2021 Sep;28 (9) :740-746.