Tubastatin A (TFA)

Tubastatin A (TSA) TFA is a potent and selective HDAC6 inhibitor with IC50 of 15 nM in a cell-free assay, and is selective (1000-fold more) against all other isozymes except HDAC8 (57-fold more) . Tubastatin A TFA also inhibits HDAC10 and metallo-β-lactamase domain-containing protein 2 (MBLAC2) .

Product Specifications

CAS Number

[1239262-52-2]

Product Name Alternative

TSA (TFA)

UNSPSC

12352005

Hazard Statement

H302-H315-H319-H335

Target

Apoptosis; Autophagy; HDAC

Type

Reference compound

Related Pathways

Apoptosis; Autophagy; Cell Cycle/DNA Damage; Epigenetics

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/tubastatin-a-tfa.html

Solubility

10 mM in DMSO

Smiles

O=C(NO)C1=CC=C(CN2C3=C(CN(C)CC3)C4=C2C=CC=C4)C=C1.O=C(O)C(F)(F)F

Molecular Formula

C22H22F3N3O4

Molecular Weight

449.42

Precautions

P261-P264-P270-P271-P280-P302+P352-P304+P340-P330-P362+P364-P405-P501

References & Citations

[1]de Zoeten EF, et al. Histone deacetylase 6 and heat shock protein 90 control the functions of Foxp3 (+) T-regulatory cells. Mol Cell Biol. 2011 May;31 (10) :2066-78.|[2]Kyle V. Butler et al. Rational Design and Simple Chemistry Yield a Superior, Neuroprotective HDAC6 Inhibitor, Tubastatin A J. Am. Chem. Soc., 2010, 132 (31), pp 10842-10846|[3]Di Fulvio S, et al. Dysferlin interacts with histone deacetylase 6 and increases alpha-tubulin acetylation. PLoS One. 2011;6 (12) :e28563|[4]Ketene AN, et al. Actin filaments play a primary role for structural integrity and viscoelastic response in cells. Integr Biol (Camb) . 2012 May;4 (5) :540-9.|[5]Brijmohan AS, et al. HDAC6 Inhibition Promotes Transcription Factor EB Activation and Is Protective in Experimental Kidney Disease. Front Pharmacol. 2018 Feb 1;9:34.|[6]Severin Lechner, et al. Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target. Nat Chem Biol. 2022 Apr 28.