Di-4-ANEPPS

Di-4-ANEPPS is a voltage-sensitive dye that acts on voltage-gated ion channels (such as sodium channels) and inhibits sodium current, significantly reducing sodium current density, although specific values like IC50 remain unclear. It mainly binds to the voltage-sensitive regions on the cell membrane, changing its fluorescence properties to reflect membrane potential changes and thus affecting the function of ion channels to exert its activity. This substance can be used in cardiovascular research, such as the electrophysiology of cardiomyocytes, myocardial ischemia, and the effects of drugs on cardiomyocytes. It is of great value in evaluating drug cardiotoxicity and exploring the mechanisms of arrhythmias[1][2].

Product Specifications

CAS Number

[90134-00-2]

UNSPSC

12171500

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/di-4-anepps.html

Purity

99.00

Solubility

10 mM in DMSO

Smiles

O=S(CCC[N+]1=CC=C(/C=C/C2=CC=C3C=C(N(CCCC)CCCC)C=CC3=C2)C=C1)([O-])=O

Molecular Formula

C28H36N2O3S

Molecular Weight

480.66

References & Citations

[1]Marina Ronzhina, et al. Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart. Front Physiol. 2021 Jun 10;12:667065.|[2]Hortigon-Vinagre MP, et al. The Use of Ratiometric Fluorescence Measurements of the Voltage Sensitive Dye Di-4-ANEPPS to Examine Action Potential Characteristics and Drug Effects on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Toxicol Sci. 2016 Dec;154 (2) :320-331.