Ponceau S

Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red[1][2][3].

Product Specifications

CAS Number

[6226-79-5]

Product Name Alternative

Acid Red 112

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/ponceau-s.html

Purity

98.0

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 35.71 mg/mL (ultrasonic)

Smiles

O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O

Molecular Formula

C22H12N4Na4O13S4

Molecular Weight

760.57

Precautions

H315, H319, H335

References & Citations

[1]Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53.|[2]Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020 (3) : 098459.|[3]Romero-Calvo I, et al. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem. 2010 Jun 15;401 (2) :318-20.