Ponceau S
Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red[1][2][3].
Product Specifications
CAS Number
6226-79-5
Product Name Alternative
Acid Red 112
UNSPSC
12171500
Hazard Statement
H315, H319, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/ponceau-s.html
Purity
98.0
Solubility
DMSO : 100 mg/mL (ultrasonic) |H2O : 35.71 mg/mL (ultrasonic)
Smiles
O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O
Molecular Formula
C22H12N4Na4O13S4
Molecular Weight
760.57
Precautions
H315, H319, H335
References & Citations
[1]Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53.|[2]Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020 (3) : 098459.|[3]Romero-Calvo I, et al. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem. 2010 Jun 15;401 (2) :318-20.
Shipping Conditions
Room Temperature
Storage Conditions
Store at room temperature, keep dry and cool
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
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