Resazurin

Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent) . Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal) [1][2].

Product Specifications

CAS Number

[550-82-3]

Product Name Alternative

Diazoresorcinol

UNSPSC

12171500

Hazard Statement

H315-H319-H335

Target

Bacterial; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Anti-infection; Others

Field of Research

Infection

Assay Protocol

https://www.medchemexpress.com/resazurin.html

Solubility

10 mM in DMSO

Smiles

O=C1C=CC2=[N+](C3=C(OC2=C1)C=C(C=C3)O)[O-]

Molecular Formula

C12H7NO4

Molecular Weight

229.19

Precautions

P261-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P362+P364-P403+P233-P405-P501

References & Citations

[1]Silva FSG, et al. Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay. Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15. |[2]Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel) . 2012;12 (9) :12347-60. |[3]Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93.