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Coelenteramine 400a (hydrochloride)

Coelenteramine (Coelenterazine) 400a hydrochloride, a derivative of Coelenterazine, is a Renilla luciferase (RLuc) substrate. In the presence of Coelenteramine 400a hydrochloride, RLuc can emit blue light at 395 nm[1][2]. Coelenteramine 400a hydrochloride will causes color change in the bioluminescence reaction of Rluc by replacing the sulfur and oxygen heteroatoms of the methylene bridge. Coelenteramine 400a hydrochloride provides higher signal resolution and can be used in the research of bioluminescence resonance energy transfer (BRET) [3].

Product Specifications

CAS Number

2320429-05-6

Product Name Alternative

Coelenterazine 400a (hydrochloride) ; Bisdeoxycoelenterazine (hydrochloride)

UNSPSC

12352005

Target

Fluorescent Dye

Type

Reference compound

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/coelenteramine-400a-hydrochloride.html

Solubility

10 mM in DMSO

Smiles

O=C1C(CC2=CC=CC=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31.[H]Cl

Molecular Formula

C26H22ClN3O

Molecular Weight

427.93

References & Citations

[1]Levi J, et, al. Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays. J Am Chem Soc. 2007 Oct 3;129 (39) :11900-1.|[2]Bertrand L, et, al. The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS) . J Recept Signal Transduct Res. Feb-Nov 2002;22 (1-4) :533-41.|[3]Yuan M L, et al. Luminescence of coelenterazine derivatives with C-8 extended electronic conjugation[J]. Chinese Chemical Letters, 2016, 27 (4) : 550-554.|[4]Ikeda Y, et al. Quantitative evaluation of luminescence intensity from enzymatic luminescence reaction of coelenterazine and analogues. Journal of Photochemistry and Photobiology A: Chemistry, 2020, 394: 112459.

Shipping Conditions

Room temperature

Scientific Category

Reference compound1

Clinical Information

No Development Reported

Frequently Asked Questions

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