4-Nitrophenyl phosphate (disodium hexahydrate)

4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

Product Specifications

CAS Number

[333338-18-4]

Product Name Alternative

P-Nitrophenyl phosphate (disodium hexahydrate)

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H332, H335

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/4-nitrophenyl-phosphate-disodium-hexahydrate.html

Purity

99.80

Solubility

DMSO : 10 mg/mL (ultrasonic; warming; heat to 80°C) |H2O : 100 mg/mL (ultrasonic)

Smiles

O=[N+]([O-])C1=CC=C(C=C1)OP(O[Na])(O[Na])=O.[6H2O]

Molecular Formula

C6H6NO6P.6H2O.2Na

Molecular Weight

371.14

Precautions

H302, H315, H319, H332, H335

References & Citations

[1]Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71 (Pt 2) :199-205. |[2]Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145 (11) :3871-3877.