What is the catalog number for CA9 Antibody?

The catalog number for CA9 Antibody is orb750277.

What sizes are available for CA9 Antibody?

CA9 Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does CA9 Antibody come in?

CA9 Antibody is available in 2 different pack sizes.

Is CA9 Antibody in stock?

CA9 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

CA9 Antibody

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200) . Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CA9 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CA9 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

UniProt

Q16790

Reactivity

Human

Immunogen

The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this CA9 antibody.

Clonality

Monoclonal

Clone

SPM314

Conjugation

Unconjugated

Field of Research

Epigenetics

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the CA9 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.