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TGF-alpha Antibody

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Product Specifications

UniProt

P01135

Reactivity

Human

Immunogen

Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for this anti-TGF alpha antibody.

Clonality

Monoclonal

Clone

SPM542

Conjugation

Unconjugated

Field of Research

Epigenetics

Purification

Precipitated followed by dialysis in 1X PBS

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgM, kappa

Available Sizes

Frequently Asked Questions

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