What is the catalog number for NKX2.2 Antibody?

The catalog number for NKX2.2 Antibody is orb750240.

What sizes are available for NKX2.2 Antibody?

NKX2.2 Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does NKX2.2 Antibody come in?

NKX2.2 Antibody is available in 2 different pack sizes.

Is NKX2.2 Antibody in stock?

NKX2.2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

NKX2.2 Antibody

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Product Specifications

UniProt

O95096

Reactivity

Human

Immunogen

Human recombinant protein was used as the immunogen for this NKX2.2 antibody.

Clonality

Monoclonal

Clone

SPM564

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1) (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the NKX2.2 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.