GCSF ADA (granulocyte colony stimulating factor) ELISA
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream
Product Specifications
Reaction Volume
15 µL
Target Antigen
antibodies to GCSF
Detection Method
Direct sandwich ELISA
Assay Principle
Quantification of antibodies to G-CSF
Assay Protocol
This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured
Assay Performance Time
2.5 Hours
Sample Type
Serum Plasma
Detection Range
45 ng/mL -1440 ng/mL
Precision
<20%, <20%
Sensitivity
anti-GCSF antibodies
Limit Of Detection
45 ng/mL
Components
Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 mL Calibrator (1.15mg/mL) 12 μl 10X wash buffer 50 mL Assay buffer 50 mL 1000X detection reagent 17 μl TMB 12 mL TMB stop solution
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Shelf Life
12 months
CAS Number
7732-18-5
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