Anti-PEG antibody ELISA (human IgM specific)
Polyethylene glycol (PEG) chains are often used to modify therapeutic biologic agents in order to prolong the circulating half-life of the modified protein. It has been reported that repeat injections of PEGylated proteins can induce anti-PEG antibodies.
Product Specifications
Reaction Volume
15 µL
Target Antigen
Anti-PEG human IgM antibodies
Detection Method
Direct sandwich ELISA
Assay Principle
Quantification of human IgM antibodies to PEG
Assay Protocol
This assay employs the sandwich enzyme immunoassay technique. Immobilized PEG is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Anti-PEG antibodies present in biological matrices is bound by the immobilized PEG. After washing away any unbound substances, enzyme linked anti IgG or IgM antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of anti-PEG antibodies present in test samples. The color development is stopped and the intensity of the color is measured
Assay Performance Time
2.5 Hours
Sample Type
Serum Plasma
Detection Range
62.5 ng/mL -1000 ng/mL
Precision
<10%, <10%
Sensitivity
Anti-PEG antibodies
Limit Of Detection
62.5 ng/mL
Components
Coated microtiter plate, 96 wells
QC samples - 6x250ul
10X wash buffer - 50 mL
Assay buffer - 50mL
1000X detection reagent - 17ul
TMB - 12mL
TMB stop solution - 12mL
Plate sealers - 3
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Shelf Life
12 months
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CAS Number
7732-18-5
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