Recombinant Villin Antibody
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Product Specifications
UniProt
P09327
Reactivity
Human
Immunogen
A portion of amino acids 600-700 from the human protein was used as the immunogen for the recombinant Villin antibody.
Clonality
Recombinant
Clone
VIL1/4107R
Conjugation
Unconjugated
Field of Research
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Purification
Protein A affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant Villin antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Rabbit
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Rabbit IgG
Available Sizes
Frequently Asked Questions
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