CD8b Antibody
This monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Product Specifications
UniProt
P10300
Reactivity
Mouse
Immunogen
Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c
Clonality
Monoclonal
Clone
H35-17.2
Conjugation
Unconjugated
Field of Research
Epigenetics, Immunology
Purification
Protein G affinity chromatography
Dilution
Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD8b antibody should be determined by the researcher.
Tested Applications
FACS, Functional Studies, IF
Host or Source
Rat
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Rat IgG2b, kappa
Available Sizes
Frequently Asked Questions
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Mouse Monoclonal IL-17B Antibody (MM0373-3D62) [PE/Cy5.5]
