CLEC9A Antibody / DNGR-1
This mAb recognizes mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31 (+) dendritic cells and on a small subset of CD14 (+) and CD16 (-) monocytes.
Product Specifications
Reactivity
Mouse
Immunogen
RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope.
Clonality
Monoclonal
Clone
1F6
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2ug/ml, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CLEC9A antibody should be determined by the researcher.
Tested Applications
FACS, IF, WB
Host or Source
Rat
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Rat IgG1, kappa
Available Sizes
Frequently Asked Questions
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