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CD3e Antibody

CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletion.

Product Specifications

UniProt

P07766

Reactivity

Mouse

Immunogen

The H2Kb-specific mouse cytotoxic T lymphocyte clone BM10 37 was used as the immunogen for the CD3e antibody.

Clonality

Monoclonal

Clone

145-2C11

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein A affinity chromatography

Dilution

Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the CD3e antibody should be determined by the researcher.

Tested Applications

FACS, Functional Studies, IF

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Armenian Hamster IgG, kappa

Available Sizes

Frequently Asked Questions

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