CD3e Antibody
CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletion.
Product Specifications
UniProt
P07766
Reactivity
Mouse
Immunogen
The H2Kb-specific mouse cytotoxic T lymphocyte clone BM10 37 was used as the immunogen for the CD3e antibody.
Clonality
Monoclonal
Clone
145-2C11
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A affinity chromatography
Dilution
Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD3e antibody should be determined by the researcher.
Tested Applications
FACS, Functional Studies, IF
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Armenian Hamster IgG, kappa
Available Sizes
Frequently Asked Questions
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