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Villin Antibody Cocktail

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Product Specifications

UniProt

P09327

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 179-311) was used as the immunogen for this Villin antibody cocktail.

Clonality

Monoclonal

Clone

VIL1/1314 + VIL1/2376

Conjugation

Unconjugated

Field of Research

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Villin antibody cocktail should be determined by the researcher.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa + Mouse IgG1, kappa

Available Sizes

Frequently Asked Questions

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