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Akt1 (phospho Tyr474) Antibody

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Swiss Prot

P31749

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Tyr474. AA range:431-480

Target

Akt1

Partner Proteins

RAC-alpha serine/threonine-protein kinase

Clonality

Polyclonal

Source

Polyclonal, Rabbit,IgG

Applications

WB, IHC, IF, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Dilution

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Modification

Phospho Specific

Storage Conditions

Store at -20°C/1 year

Specificity

Phospho-Akt1 (Y474) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at Y474.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Available Sizes

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