Clathrin Heavy Chain Antibody
Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.
Product Specifications
UniProt
Q00610, P53675
Reactivity
Human
Immunogen
Recombinant full-length human Clathrin Heavy Chain protein was used as the immunogen for this Clathrin Heavy Chain antibody.
Clonality
Monoclonal
Clone
CHC/1432
Conjugation
Unconjugated
Field of Research
Autophagic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Clathrin Heavy Chain antibody should be determined by the researcher.
Tested Applications
IF
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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