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CHD4 Antibody / Chromodomain helicase DNA binding protein 4

CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Product Specifications

UniProt

Q14839

Reactivity

Human, Mouse

Immunogen

Amino acids ASGLGSPSPCSAGSEEEDM and CSRLANRAPEPPPQQVAQQQ were used as the immunogen for the CHD4 antibody.

Clonality

Monoclonal

Clone

3F2/4

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Protein G affinity chromatography

Dilution

ELISA (order BSA-free format for coating), Western blot: 1-2ug/ml, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the CHD4 antibody should be determined by the researcher.

Tested Applications

ELISA, IF, IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2a, kappa

Available Sizes

Frequently Asked Questions

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