Recombinant Nuclear Marker Antibody

This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells.

Product Specifications

Reactivity

Human

Immunogen

Nuclei of HL60 cells were used as the immunogen for the recombinant Nuclear Marker antibody.

Clonality

Recombinant

Clone

NM2984R

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant Nuclear Marker antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.