Recombinant CD81 Antibody

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1) . CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Product Specifications

UniProt

P60033

Reactivity

Human

Immunogen

A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the recombinant CD81 antibody.

Clonality

Recombinant

Clone

C81/2885R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein A affinity chromatography

Dilution

ELISA: order BSA/sodium azide-free format for coating, Immunohistochemistry (FFPE) : 1-2ug/ml, Flow cytometry: 0.5-1ug/million cells

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.