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Recombinant NF-L Antibody / Neurofilament Light

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]

Product Specifications

UniProt

P07196

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant NF-L antibody.

Clonality

Recombinant

Clone

MSI27-2R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein A affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant NF-L antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Rabbit

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Rabbit IgG, kappa

Available Sizes

Frequently Asked Questions

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