Recombinant CD20 Antibody / Rabbit Monoclonal
CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells. [Wiki]
Product Specifications
UniProt
P11836
Reactivity
Human
Immunogen
Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
Clonality
Recombinant
Clone
RMC20-1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Tested Applications
FACS, IHC-P
Host or Source
Rabbit
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Rabbit IgG, kappa
Available Sizes
Frequently Asked Questions
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